Abstract

EFFECT OF TERMINALIA BELERICA FRUITS ON STREPTOZOTOCIN-INDUCED DIABETES MELLITUS AND ITS ASSOCIATED COMPLICATIONS

Arvind Mishra, Sudeep Gautam, Savita Pal, Akansha Mishra, Rakesh Maurya, Arvind Kumar Srivastava*

Abstract

The present study investigated the effect of extracts and fractions of Terminalia belerica fruit extracts on hyperglycemia and associated complications on streptozotocin-induced diabetic rats. The aqueous extract and 95% and 50% ethanol extracts were administered to the normal rats with oral sucrose load and streptozotocin-induced diabetic rats. The extracts significantly decreased hyperglycemia in streptozotocin-induced diabetic rats and also showed lowering of postprandial hyperglycemia in normal rats. Based on antihyperglycemic activity profile the 95% ethanol extract was further fractionated to obtain hexane, chloroform, butanol and aqueous fractions which were administered to STZ-induced diabetic rats. All except aqueous fraction showed significant antihyperglycemic activity and the maximum activity was registered in butanol fraction treated group. The butanol fraction also exhibited significant effect on various parameters in diabetic animals such as lipid profile, serum insulin level, glycated hemoglobin, hepatic and renal function markers. It also enhanced glucose uptake in a concentration-dependent manner in L6 myotubes and stimulated the tyrosine phosphorylation of IRS-1, AKT and GLUT-4 at both mRNA and protein level. The findings suggest that the butanol fraction of 95% ethanol extract of Terminalia belerica fruits is competent in combating various symptoms and complications of diabetes mellitus.

Keywords: Hyperglycemia, diabetes, T.belerica, streptozotocin, high-fructose diet.