Abstract

EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF SEED EXTRACT OF CLITORIA TERNATEA AND OCIMUM SANCTUM ON CARRAGEENAN INDUCED PAW EDEMA IN RATS

Yashsvee Verma, Anchal Verma*

Abstract

Herbal medicines have long been used in traditional systems of healthcare for the treatment of inflammatory disorders due to their safety and efficacy. This study evaluates the anti-inflammatory activity of ethanolic seed extracts of Clitoria ternatea and Ocimum sanctum using the carrageenan-induced paw edema in rats. Both plants were extracted in ethanol. Extracts were administered orally at 200 mg/kg using 2% gum acacia as a suspending agent. Animals were divided into seven groups control, standard, individual extracts, and combination treatments of CTSE and OSSE in 1:1, 1:2, and 2:1 ratios. All treated groups showed significant reduction in paw edema (p < 0.01), with the 2:1 CTSE: OSSE combination exhibiting the highest anti-inflammatory activity, exceeding that of Diclofenac. GC-MS analysis revealed the presence of several phytoconstituents in the extracts, suggesting their role in mediating the observed effects. These findings indicate a synergistic interaction between CT and OS seed extracts.

Keywords: Clitoria ternatea, Ocimum sanctum, GC-MS, Anti-inflammatory activity, Carrageenan, Diclofenac, Plethysmometer.