Abstract

APPLICATION OF TWO NOVEL SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF LOSARTAN POTASSIUM AND CHLORTALIDONE IN PHARMACEUTICAL DOSAGE FORM

Soniya A Thomas* and Mary Mathew

Abstract

Objective: To develop two novel, simple, specific and accurate spectrophotometric methods for simultaneous estimation of Losartan Potassium and Chlortalidone without prior separation steps. Methods: Ratio difference spectrophotometric method (RDSM) and mean centering of ratio spectra method (MCR). Results: Calibration curves of the two methods are linear over the concentration range of 10-40μg/ml and 10-60μg/ml for Losartan Potassium and chlortalidone respectively, with good correlation coefficients. The methods were validated as per ICH guidelines; accuracy, precision and repeatability are found to be within acceptable limit. Conclusion: The two novel spectrophotometric methods are simple, accurate, precise, reproducible, economic and valid for application in laboratories lacking liquid chromatographic instruments.

Keywords: Losartan Potassium, Chlortalidone, ratio difference spectrophotometric method, mean centering of ratio spectra.