Abstract

EVALUATION OF WOUND HEALING ACTIVITY OF LEAF OF BARLERIA LUPULINA

Ajay Mandal*, Mrunal Sene P and Sandeep Kumar Dey

Abstract

Barleria lupulina belongs to the family Acanthaceae and is a large, widespread, polymorphic, pantropical genus of herbs and shrubs comprising some 300 species are used used traditionally for the treatment of variety of diseases including anaemia, toothache, coughs and as a hypoglycemic agent. The present study was aimed at evaluation of wound healing activity of the plant. In the present study the roots of Barleria lupulina were studied for wound healing activity by incorporating the methanolic and the total aqueous extract in simple ointment base B.P. in concentration of 0.5% (w/w), 1% (w/w) and 2% (w/w).Wound healing activity was studied in three types of model in rats viz. excision, incision and estimation of biochemical parameter. In case of the excision wound model wound contraction and period of epithelisation was studied while in incision wound model was evaluated by determining tensile strength and hydroxyproline content in the scab. Treatment of wound with ointment containing 2% (w/w) the methanolic and 2% (w/w) the total aqueous extract exhibited significant (P < 0.001) wound healing activity. The methanolic and total aqueous extracts were analyzed for total phenols content equivalent to Gallic acid. The content of total phenols was 10% (w/w) and 16% (w/w) in methanolic and total aqueous extract respectively.

Keywords: Barleria lupulina, hypoglycemic, Acanthaceae, epithelisation,