Abstract

IMPACT OF COLEUS AROMATICUS EXTRACT ON CCL4 -MEDIATED OXIDATIVE DAMAGE IN RAT LIVER

Dr. Jayachitra J.*, Dr. Chitra M. and Elakkiya S.

Abstract

The antioxidant effect of the ethanolic extract of Coleus aromaticus, an indigenous ayurvedic medicinal plant used in India, was studied in rats with CCl4-induced hepatotoxicity. Rats were divided into four groups: Groups 1 received control, Groups 2 received CCl4, Groups 3 received CCl4 and silymarin and Groups 4 received CCl4 and ethanolic extract of Coleus aromaticus for 14 days. The results showed significantly elevated level of serum and tissue thiobarbituric acid reactive substances, and significantly lowered activities/levels of antioxidants such as superoxide dismutase, catalase, glutathione-S-transferase, glutathione reductase, glutathione peroxidase and reduced glutathione in CCl4-treated rats compared with control rats. Administration of ethanolic extract of Coleus aromaticus to rats with CCl4-induced liver injury significantly decreased the levels of serum and tissue thiobarbituric acid reactive substances and significantly elevated the activities of superoxide dismutase, catalase, glutathione-S-transferase, glutathione reductase, glutathione peroxidase and reduced glutathione in the tissues compared with unsupplemented CCl4-treated rats. These findings suggest that ethanolic extract of Coleus aromaticus has a modulatory effect on CCl4-induced hepatotoxicity in rats.

Keywords: Antioxidant, Coleus aromaticus, lipid peroxidation and CCl4.