Abstract

POTENTIAL ANTITUMOR AND ANTIOXIDANT EFFECT OF GARLIC (ALLIUMSATIVUM) OIL IN FEMALE MICE INJECTED WITH EHRLICH ASCITES CARCINOMA CELLS

Gehan Salah Eldin Moram*, Nahla Hussein Ali, Ola Mohamed Samy Ahmad Sadek 

Abstract

Objectives: This study was undertaken to investigate the antitumor and antioxidant tpotential of garlic oil (GO)in vivo using Ehrlich Ascites Carcinoma (EAC) cells. Methods: Female Swiss albino mice were divided into 6 groups: G1: (Healthy control), G2:(Healthy GO) received oral dose of GO (100 mg/Kg/day), G3: (Tumor bearing mice TBM) mice were injected intramuscularly with (2.5 x 106 EAC/ml) to form solid tumors. G4:(GO Protection)mice were orally pretreated with GO(100 mg/Kg/day) for two weeks then injected with (2.5 x 106 EAC/ml), G5:(GO Treatment) mice were injected with (2.5 x 106 EAC/ml), then treated with GO(100 mg/Kg/day) for two weeks andG6: GO(Protection + Treatment)mice were pretreated with GO(100 mg/Kg/day) for two weeks, then injected with (2.5 x 106 EAC/ml) and continued to receive GO at the same dose. Results: The antitumor potential of GO was evident through the significant prolongation of lifespan of (TBM); up to (53.57%),regression in tumor growth rate(-36.38 %) and inhibition of histonedeacetylase enzymes (-12%). GO significantly elevated the depleted oxidative stress markers including (TAC), (GSH), (GST) and (CAT) and also significantly reduced the marked elevation of malondialdehyde level in TBM (-33.7%).Moreover, GO significantly improved liver function and hematological parameters in TBM. These results were confirmed by histopathological examination of the liver. In conclusion, GO administration significantly inhibited tumor growth and histonedeacetylation. Also, GO attenuated the severity of oxidative damage accompanying tumor development, all while exercising meyloprotectiveand hepatoprotectiveproperties.

Keywords: Garlic oil, EAC, antioxidant, HDAC, epigenetic, tumor bearing mice.