Abstract

EFFECT OF PLGA-CHITOSAN-TWEEN 80 NANOPARTICLES ON BEHAVIOR AND HISTOPATHOLOGY OF HIPPOCAMPUS OF WISTAR RATS

Kinjal C Patel* and Dr. Divakar Goli

Abstract

Present study aimed to evaluate effect of PLGA-Chitosan-Tween 80 nanoparticles on the behavior and histology of hippocampus of Wistar rats designed to ascertain whether the nanoparticles improve memory, cognitive deficits and prevent degeneration of hippocampus region of rat brain under AD. This research was carried out using 40 Wistar rats. The animals were divided into four groups. Group I control, II positive control, III standard (drug treated) and IV PLGA-CS-Tween 80 NPs treated group. Animals were evaluated for behavioral study on 0, 7, 14, 28, 36 and 42th day by elevated plus maze paradigm. After 42th day, animals were humanly sacrificed using chloroform and then the brain tissues were fixed immediately in Bouin’s fluid and processed further through the routine tissue processor. The stained samples were examined by means of light microscope for histological changes. Behavioral study demonstrated that Retention Transfer Latency (RTL) was decreased in the groups IV and III gradually compare to group II rats. Prepared nanoparticles have been demonstrated improvement in memory and cognitive functions like standard drug in behavioral study. Histology examination showed loosely packed pyramidal cells, neuritic plaques and neurofibrillary tangles, which were indications of neurodegeneration in the group II. However, the group III and IV showed tightly packed pyramidal cells, less number of neuritic plaques and neurofibrillary tangles, which concluded that PLGA-Chitosan-Tween 80 NPs have significant effect on memory, cognitive functions and histopathology compared to group II. The behavioral study and histopathology study supports the performance of NPs in vivo into AD model.

Keywords: PLGA-Chitosan-Tween 80 nanoparticles, Alzheimer’s disease, behavioral study, Histopathology, Brain targeting.