Abstract

ANTIBIOFILM EFFECT OF IRON OXIDE NANOPARTICLES SYNTHESIZED BY LACTOBACILLUS FERMENTUM ON CATHETER

Jehan A. S. Salman*, Mohammed F. Al –Marjani, Raghad A. Abdulrazaq, Inas A. S Salman and Nawar B. Kamil

Abstract

The iron oxide nanoparticles have been synthesized by Iraqi isolate of Lactobacillus fermentum. Nanoparticles were characterized by Atomic Force Microscope (AFM) and found the average size 68.28 nm. Antibacterial activity of the Iron Oxide nanoparticles were investigated against some pathogenic bacteria by using co culture technique ,the results showed that iron oxide nanoparticles had inhibitory effect against pathogenic bacteria with reduction of growth reached to (46,35,30)% for Staphylococcus aureus, Escherichia coli and Serratia marcescens respectively. The effect of iron oxide nanoparticles combined with different antibiotics was investigated against pathogenic bacteria using disk diffusion method, the results showed the antibacterial activities of some antibiotics like cephalexin and Nalidixic acid have been increased in the presence of iron oxide nanoparticle against E.coli. Anti biofilm effect of iron oxide nanoparticles on coated catheters was observed against S. aureus and E.coli, iron oxide nanoparticles recorded maximum biofilm inhibition 33.97% against S. aureus, followed by 16.92% occurred against E.coli, While no inhibition on biofilm formation of S.marcescens was observed.

Keywords: Nanoparticles, Iron oxide, biosynthesis, Lactobacillus, Antibiofilm.