Abstract

DECIPHERING THE SHIELD: CALLISIA REPENS LEAF EXTRACT ATTENUATES OXIDATIVE STRESS AND HEPATIC INJURY IN EXPERIMENTAL RAT MODELS

Sajid Miya, M. Srinivasa Murthy, Saritha Kodithala*, Mohammad Mansoor, Ch. Sampath Kumar, Ashok Kumar Uppuluru

Abstract

The present study investigates the pharmacological, antioxidant, and hepatoprotective activities of Callisia repens leaves extracted in experimental rats. The plant material was collected, authenticated, dried, and extracted using the Soxhlet extraction method, followed by percentage yield determination. A phytochemical screening confirmed the presence of flavonoids, phenolics, alkaloids, tannins, terpenoids, and saponins, which are known for their medicinal properties. The antioxidant activity was assessed using the DPPH radical scavenging assay, which demonstrated a strong free radical neutralization capacity, indicating its potential in oxidative stress management. The FTIR study identified key functional groups contributing to its biological activity. The acute oral toxicity study revealed that the extract is safe at therapeutic doses. For the hepatoprotective study, hepatotoxicity was induced in rats using Paracetamol, and the protective effects of C. Repens extract were evaluated through biochemical parameters including ALP, SGOT, SGPT, and total bilirubin levels. The treatment significantly improved these markers, suggesting its hepatoprotective potential. Histopathological analysis further confirmed reduced hepatic inflammation and cellular damage in extract-treated groups compared to control groups. The study demonstrates that C. Repens possesses potent antioxidant and hepatoprotective properties, which can be attributed to its bioactive constituents. The findings suggest its potential use as a natural therapeutic agent for oxidative stress-induced liver diseases, though further clinical research is needed for validation.

Keywords: Callisia repens, Hepatoprotective activity, antioxidant activity, FTIR, phytochemical screening.