Abstract

SPECTROPHOTOMETRIC DETERMINATIONOF DRUGS USING NBROMOSUCCINIMIDE AND RHODAMINE-B DYE AS ANALYTICAL REAGENT

Tirupathi Baddenapalli and Venkateshwarlu Gandu*

Abstract

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Tramadol hydrochloride, Dobutamine hydrochloride, Trimetazidine, Terazosin hydrochloride dihydrate and Esmolol hydrochloride, based on their reactivity towards N- bromosuccinimide (NBS). The methods involve the addition of excess NBS of known concentration in the presence of 1M HCl, reactants are allowed to react and the unreacted NBS is estimated by using Rhodamine-B dye (λmax 557nm). These methods have been applied for the determination of drugs in their pure form as well as in tablet formulations. The effect of excipients has also been studied and found to have no effect. These methods have been validated in terms of guidelines of ICH.

Keywords: Spectrophotometry, Drugs, NBS, Rhodamine-B, Quantification, Validation.