Abstract

STANDARDIZATION AND PHYTOCHEMICAL PROFILING OF KIRUBHAKADATCHADHIRAVAGAM USING PHYSICOCHEMICAL PARAMETERS, GC–MS AND HPTLC FINGERPRINTING

V. Sandhiya*, K. Shyamala, T. Maharasi Maniselvi, A. Anbumalar

Abstract

Traditional Siddha formulations require systematic scientific validation to ensure their quality, safety, and reproducibility. The present study focuses on the standardization of Kirubha Kadatcha Dhiravagam (KKD), a classical Siddha preparation, using physicochemical evaluation, preliminary phytochemical screening, gas chromatography–mass spectrometry (GC–MS), and high-performance thin layer chromatography (HPTLC) fingerprinting. Physicochemical parameters such as pH, specific gravity, and total solids were analyzed to assess the formulation’s quality and stability, while preliminary phytochemical screening was carried out to identify major classes of bioactive constituents. Advanced analytical techniques, including GC–MS, were employed to detect and characterize chemical constituents, and HPTLC fingerprinting was performed to establish a chromatographic profile for the formulation. This study aims to develop a comprehensive standardization protocol for KKD that can serve as a reference for quality control, ensure batch-to-batch consistency, and support further pharmacological and clinical investigations of Siddha formulations.

Keywords: Siddha medicine, Kirubha Kadatcha Dhiravagam, standardization, HPTLC, GC–MS, phytochemical analysis.