Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DEFERIPRONE IN BULK AND TABLET DOSAGE FORM BY USING RP-HPLC METHOD

Dr. Madireddy Mamata*, Thalari Akshaya, Mir Ahmed Talha, Dolakala Akshaya, Veerangoni Harika, Kukka Vennela

Abstract

High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of Deferiprone was done by RP-HPLC. The chromatographic conditions were effectively created for the partition of Deferiprone by utilizing INERTSIL column, C18(150×4.6 ID). The detection was carried out using UV detector at 280nm.The solutions were chromatographic at a constant flow rate of 1.0ml/min. Linear regression coefficient was not more than 0.994. The accuracy study was precise, robust and repeatable. LOD esteem was 22.93 μg/ml. and LOQ esteem was 96.37 μg/ml. The results obtained on the validation parameters met ICH and USP requirements it inferred the method found to be simple, accurate, precise and linear. The method was found to be having suitable application in routine laboratory analysis with high degree of accuracy and precision.

Keywords: INERTSIL C18 column, Deferiprone, RPHPLC.