Abstract

COMBATING MULTI-DRUG RESISTANCE IN E. COLI AND S. AUREUS WITH METHANOLIC FLOWER EXTRACTS OF SPILANTHES OLERACAE AND ESTIMATING ITS PHYTOCHEMICAL CONSTITUTES

Syed Mohd. Danish Rizvi, Sibhghatulla Shaikh, Shashank Kumar Sharma, Shazi Shakil*, Adel M. Abuzenadah, Hamida Aaqil, Dinesh Chandra Sharma, Salman Khan, Manaal Zahera, Rohit Kr. Tiwari

Abstract

Background and Purpose: At present, combating multidrug resistance in bacteria remains a major challenge globally. There is an urgent need to replace currently available antibiotics. However, plant based drugs have been proved to be an effective alternative for the treatment of infectious diseases caused by bacteria. In the present study, we have evaluated the antibacterial activity of methanolic extract of flower of Spilanthes oleraceae by using agar well diffusion and serial micro dilution method against drug resistant strains of Staphylococcus aureus and Escherichia coli. Methods: The antioxidant activity of the crude extract was determined by using the DPPH method. Further, phytochemical investigation was done by GC-MS analysis. Results: Remarkable inhibition of the bacterial growth was observed against the tested organisms and minimum inhibitory concentration (MIC) of crude extract of S. oleraceae flower was found to be 0.75 and 0.25 mg/ml against S. aureus and E. coli, respectively. The antioxidant results indicated that crude methanolic extract exhibited DPPH radical scavenging activity comparable to ascorbic acid (IC50 of ascorbic acid = 0.25 mg/ml; IC50 of crude extract = 0.27 mg/ml). Moreover, GC-MS results showed that the major groups present in the extract are aromatic (50%) and aliphatic compounds (30%). The presence of wide range of phytochemicals in the methanolic extract of S. oleraceae, might be considered responsible for its antibacterial and antioxidant potential. Conclusions: Thus, crude extract of this plant can be used to discover bioactive natural compounds that may serve as leads in the development of novel agents of biomedical importance especially against multidrug resistant E.coli and S. aureus.

Keywords: Multi drug resistance, Spilanthes oleraceae, Antibacterial, Antioxidants, Bioactive compounds, GC-MS.