Abstract

ANTI TUBERCULOSIS ACTIVITY AND GC-MS ANALYSIS OF ETHYL ACETATE EXTRACTS OF WRIGHTIA TINCTORIA BARK

*Rajendra Singh, Arun Kakkar Vinod Kumar Mishra

Abstract

Wrightia tinctoria (WT) is a known rich medicinal plant in India. In this study, bioactive compounds of WT bark were extracted; isolated and evaluated. Dried WT bark was successively extracted with different solvents in Soxhlet apparatus. All the extracts were in vitro tested for their potential against M. tuberculosis. Promising extracts were subjected to column with for the fractionation of the compounds and were analyzed by GC-MS. Ethyl acetate extract showed MIC 6.25 μg/ml against M. tuberculosis which was significantly different from other extract of WT bark. Proximate analysis results showed the presence of Alkaloids, Carbohydrates, Proteins, Glycerol, Resins, Tannins, Sterols Cardiac Glucosides and Triterpinse. Chromatogram was analyzed and matched with NIST library. As per the matching, Methoxyacetic acid 3-tridecyl ester, Quinoline, 2,7-dimethyl, Hexadecane , 4,5-dimethyl pentadecane, Nonadecane and Docosane were identified in fraction 1. Similarly in fraction 2, 3, 4 and 5 peaks were analyzed and characterized as Benzene,1,3-dichloro, Dodecafne, Benzothiazole, Tetradecane, Ethano l,2-(Octadecyloxy), Octadecane, Dibutyl phthalate, Eicosane, Benzene 1,3-dinitro, Heptadecane 2,6,10,15-Tetramethyl, 1-Hexadecanol, Tridacane-4-methyl, Phthalic acid butyl, 2- pentyl ester and Erucic acid presence was confirmed. Current study is focused on characterization of constituents responsible for the anti-tuberculosis activity of the plant.

Keywords: Bioactive compound, ethanomedicine, tuberculosis.