Abstract

PHARMACOGNOSTIC CHARACTERIZATION, PHYSICOCHEMICAL STANDARDIZATION, AND PHYTOCHEMICAL PROFILING OF THE WHOLE PLANT OF ORTHOSIPHON THYMIFLORUS (ROTH) SLEESEN (LAMIACEAE)

Saran Mohan M. B.^*, Harigovind M., Visakh V. V., Elizabeth M. Abraham, Simi S. M.

Abstract

Orthosiphon thymiflorus (Roth) Sleesen (Lamiaceae), locally known as "Kattuthritha" or "Neer-naadan," is a perennial herb valued in South Indian and Southeast Asian traditional medicine for its diuretic, anti-inflammatory, and anti-diabetic properties. Despite its ethnopharmacological relevance, comprehensive pharmacognostic standards for this species are lacking, hindering quality control and authentication. This study addresses this gap by establishing detailed pharmacognostic, physicochemical, and phytochemical profiles for the whole plant. Authenticated plant material underwent macroscopic and organoleptic evaluation, microscopic analysis of leaf, stem, and root transverse sections, and powder microscopy. Physicochemical parameters were determined per WHO guidelines. Preliminary and quantitative phytochemical analyses were performed on chloroform and ethanolic extracts. Macroscopic findings confirmed characteristic Lamiaceae features: quadrangular stems and opposite, ovate-lanceolate, serrate leaves. Microscopy revealed a dorsiventral leaf structure, diacytic stomata, glandular trichomes, and collateral vascular bundles. Physicochemical values included total ash (8.5% w/w), acid-insoluble ash (2% w/w), water-soluble extractive (4% w/w), alcohol-soluble extractive (2% w/w), and loss on drying (1.29 g). Phytochemical screening identified phenols, flavonoids, and triterpenoids, with ethanolic extracts exhibiting a richer profile. These established botanical, physicochemical, and phytochemical parameters provide a crucial reference standard for the accurate identification, quality control, and future therapeutic development of Orthosiphon thymiflorus, ensuring its differentiation from related taxa and preventing adulteration.

Keywords: Orthosiphon thymiflorus, Lamiaceae, Pharmacognosy, Powder Microscopy, Physicochemical Standardization, Phytochemical Screening, Quality Control.