Abstract

METHOD DEVELOPMENT AND SIMULTANEOUS ESTIMATION FOR SKELETAL MUSCLE RELAXANT IN SOLID DOSAGE FORM USING RP-HPLC

*Ms. Shraddha Lunge

Abstract

A reversed-phase liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of tolperisone hydrochloride (TOLP) and etodolac (ETD) in a solid dosage form. The analysis was performed using a Grace C18 column. The optimal composition of the mobile phase was found to be Methanol: Buffer 55:45 (v/v). The flow rate was set at 1.4 ml/min and UV detection was carried out at 254 nm. Retention times of Etodolac 8.30 and Tolperisone (9.36). The method showed a good linearity range between 10-50ug/ml for etodolac and 2-10 ug/ml for tolpresione. The LOD was found to be 0.17µg/mL and 0.08µg/mL for Etodolac and Tolperisone respectively and LOQ was found to be 0.51µg/mL and 0.25µg/mL for Etodolac and Tolperisone respectively. % Recovery study for Etodolac and Tolpresione was found to be 100.57,99.94 respectively. The RSD for the precision of the method was found to be less than 2%.

Keywords: Etodolac, tolperisone, simultaneous estimation, validation.