Abstract

PHARMACOLOGICAL EVALUATION OF STEM BARK EXTRACT OF THESPESIA POPULNEA (MALVACEAE) FOR ANALGESIC ACTIVITY IN MICE

Veerendra V.* and Sai Manohar V.

Abstract

Objective: To determine the analgesic activity of stem bark extract of Thespesea populnea in Swiss albino mice. Method: For this study 60 Swiss albino mice of either sex were randomly selected and divided into 10 equal groups. Assessment of analgesic activity was done by using Hot plate method after administration 0.5ml gum acacia to Group 1, Pentazocine 25mg/kg to group 2, and group 3, 4 & 5 received 3, 10, and 30mg/kg stem bark extract of Thespesea populnea (TP) respectively. The latent period, jumping or licking response was noted. Acetic acid induced writhing was done in mice of groups 6 to 10 after administration of 0.5ml gum acacia to Group 6, Diclofenac sodium 10mg/kg to group 7 and group 8, 9 & 10 received 3, 10, 30mg/kg stem bark extract of TP respectively. The onset, duration& number of writhing in mice was noted. The results were analysed statistically. Result: In hot plate method TP (30 mg/kg) the % increase in jumping response was more significant compared to standard drug pentazocine (25 mg/kg) and TP (30 mg/kg) gave the peak effect at 60 minutes whereas pentazocine showed a peak response at 180 minutes. In acetic acid induced writhing test, the % inhibition up to 20 minutes for the TP (30 mg/kg) was 68% and for diclofenac the % inhibition up to 20 minutes was 80%. Conclusion: Thespesea populnea had shown good analgesic effect in central as well as peripheral models.

Keywords: Thespesea populnea, Hot plate method, Writhing test, Analgesic activity.