Abstract

TO EVALUATE ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITY OF EXTRACTS OF C. TERNATEA

Jyotsna V. Phulbandhe*, Sujata Deo, Farhin Inam and Pravin K. Bhoyar

Abstract

The works aims to evaluate antimitotic and antiproliferative activity of extracts of C. Ternatea. The work exploits the antioxidant potential using DPPH radical Scavenging method and nitric oxide radical scavenging method. The antimitotic activity was screened using Allium cepa root meristamatic cells which have been used extensively in screening of drug with antimitotic activity. The anti-proliferation activity was evaluated using Yeast as a model system.Percentage of Mitotic index for the test extracts ACCT and MECT was found to be 35.84 % and 46.87 % respectively. ACCT was found to be most potent than other extracts and also appeared significantly better than that of standard, Methotrexate with 43.05 %. The anti-proliferative activity of the selected extracts was observed in decreasing order of ACCT > MECT. ACCT appeared most potent with 49.60 % and MECT with 30.56 % inhibition respectively at 100 mg/ml. The promising antimitotic and anti-proliferative activity of ACCT can be directly correlated with the presence of higher amount of total flavonoids and polyphenols. It is evident from these studies that successive extraction of these plants lead to segregation of biomolecules and degree of polymerization plays an important role in determining the pharmacological potential of extracts and isolated molecules.

Keywords: C. ternatea, flavonoids, DPPH and nitric oxide.