Abstract

BUTACHLOR INDUCED OXIDATIVE AND HORMONAL IMBALANCE CORRELATED WITH OVARIAN ANOMALIES IN FRESH WATER INDIAN CAT FISH CLARIAS BATRACHUS (LINN.): SEM STUDY

Prakriti Verma* and G. B. Chand

Abstract

Fresh water Indian cat fish Clarias batrachus (Linn.) were exposed to Butachlor 2 chloro-N-(2-6 diphenyl) acetamide exposure at a dose of 1.5μl/L for 5, 10 and 15 days respectively. At the termination of each exposure blood samples were collected and analysed for serum Lipid peroxides (LPO), reduced glutathione (GSH), Thyroid stimulating hormone (TSH) and Estradiol level as per standard method of WHO. The ovarian tissues were fixed in chilled (4ºC) 2.5% gluteraldehyde followed by its post fixation in 1% OSO4 in 0.1 M Phosphate Buffer at pH 7.2. The tisssues were further processed for SEM studies. The results showed a significant (p

Keywords: Butachlor, Clarias batrachus, Estradiol, GSH, LPO, Ovarian tissues, SEM, TSH.