Abstract

ISOLATION, SCREENING CHARACTERIZATION, OF PGPR AND THEIR ANTAGONISM TEST AGAINST ALTERNARIA SOLANI

Upasana, Shukla Lokesh, Dr. Minakshi Dwivedi*

Abstract

Plant growth promoting rhizobacteria (PGPR) are a group of beneficial bacteria that can be found in the rhizosphere, in association with root which can enhance the growth of plant directly or indirectly by several mechanisms. The use of PGPR is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticide and supplements. In the present study, A total six bacterial isolates in vitro screening was done for different plant growth promotion activities i.e. phosphate solublization, IAA production, Siderophore production. Six isolates of bacterial, designated as TR25, TR21, TR22, TR23, TR24, and TR15, were successfully isolated and characterized. In the present work five bacterial isolates were positive for IAA production and siderophore production and one (TR25) isolates were found to be positive for phosphate solublization. Most of the isolates grown best under the temperature of 200C & 280c when compared to 100c & 370C and very few can grown at 450c, Furthermore, most of the PGPR isolates shown antifungal activity against Alternaria solani. The present study, there for, suggest that the use of PGPR isolates TR25, TR21, TR15, and TR22 as inoculants /biofertilizers might be beneficial for tomato cultivation as they enhanced growth of tomato due to production of Indole acetic acids, phosphate solublization and also having antifungal activity against phytopathogenic fungi.

Keywords: Plant growth promoting rhizobacteria, antagonism assay, siderophore production, phosphorous production, IAA production.