Abstract

EFFECT OF PHYSICAL MUTAGENESIS ON THE ABILITY OF STAPHYLOCOCCUS AUREUS IN STAPHYLOKINASE PRODUCTION

Hameed M. Jasim, Riad A. Dellol and Alaa S. Hamzah

Abstract

This study included collecting isolate S. aureus, a total of (200) clinical samples were collected from two clinical sources were (100) samples of urine and (100) samples from skin infections. Staphylokinase production by S.aureus carried out , ability of (54) isolates S.aureus were tested to staphylokinase production in selective medium plasma agar and the results showed all isolates were produced staphylokinase in different levels and appered zone of lysis on plasma agar and chose efficient producing isolate was (S.aureus A15) for further experiments. Enhanced staphylokinase production by UV mutated (S.aureus A15) achieved after exposure fresh culture of S.aureus A15 for UV irradiation (254nm) for 30 second under sterile conditions and the results appeared two mutated isolates were more production comparison with wild type. Genomic DNA of the all isolates S.aureus before and after mutagenesis were extracted by boiling method, and amplification of Sak gene by PCR carried out by specific primers and size of staphylokinase gene was about 400bp.

Keywords: S. aureus, staphylokinase, S.aureus A15.