Abstract

“EMBEDDING POTENTIAL BIOINOCULANT STRAIN FOR EFFECTIVE SOIL CONDITIONER PRODUCTION”

Sumant D. Lad*, Arti Shanware and Minal Trivedi

Abstract

Biofertilizer is the resource and producer of plant nutrients and promotes growth by increasing supply of essential nutrients and growth induced to the target crop when applied to plant surfaces, seed and soil. Biofertilizers are environmental friendly fertilizers that aid in Nitrogen fixation, Phosphate solubilization and Potassium mobilization. But studies regarding organisms solubilizing secondary nutrients like sulphur and magnesium are yet to be carried out optimally. Sulphur and Magnesium are secondary nutrients required in small quantities. In the present investigation Sulphur oxidizing bacteria(SOB) and Magnesium oxidizing bacteria (MOB) were isolated from Sugarcane(Ladewadgaon, Dist.Beed, Maharashtra) & Citrus rhizosphere (Rajiv Gandhi Biotechnology Centre, RTMNU, Nagpur). The isolates were characterized by using Morphological and biochemical, cultural tests and screened by PCR using specific oligonucliotide primers. Isolates were sequenced by 16S r RNA sequencing for molecular characterization and identification. Bacillus sp. & Serratia marcescens sp. were found to be competent isolates for sulphur & magnesium oxidation respectively. Further green house studies were conducted to see their impact of plant growth.

Keywords: Biofertilizers, Sulphur, Magnesium, Secondary, PCR, 16s r RNA.