Abstract

SPECTROSCOPIC AND ELECTROCHEMICAL STUDIES ON THE DNA BINDING STUDIES OF SOME ASYMMETRIC COMPOUNDS

Shaista Sabir*, Naghmana Rashid and Maryam Zaheer

Abstract

DNA binding behavior of four asymmetric compounds has been investigated by UV-Visible spectroscopy and cyclic voltammetry. Calf Thymus DNA was used for interaction at blood pH of 7.4. Four compounds A-D [4-Hydroxy-3- (3-oxo-1-phenylbutyl) -2H-chromen- 2-one], [4-Hydroxy-3- (3-oxo-1,3-diphenylpropyl) -2H-chromen-2- one], [4-Hydroxy 3-[1-(4 Bromophenyl) -3-oxo-3-phenylpropyl] -2Hchromen- 2-one] and [4-Hydroxy-3- [1-(4-bromophenyl) -3-oxo-3- phenylpropyl] -2H-chromen-2-one] are asymmetric compounds and are the derivatives of 4-Hydroxycoumarin. From UV and CV studies, the binding constants Kb and free energy ΔG were calculated. All the studied compounds showed weak to moderate interactions with DNA. UV results showed that compound A interacted with DNA through electrostatic interactions, B and C through groove bindings while compound D showed typical intercalation mode. Same response was observed after CV results.

Keywords: Coumarins, interactions, groove bindings, intercalation.