Abstract

DIAGNOSIS OF ENTEROBACTER SAKAZAKI FROM SAMPLES OF INFANT MILK, STOOL AND HABOUBI HOSPITAL ENVIRONMENT IN DHI QAR PROVINCE AND STUDY THE SENSITIVITY FOR SOME ANTIBIOTICS

Amany Shakeir Jaber* and Bushra Jabbar Al. Badry and Murtada Hafedh Hussein, Intidhaar N. Abid

Abstract

This study was aimed for detection occurrence of Enterobacter sakazakii in stool specimens, infant formula and hospitals environments. A total of 310 samples (50 infant formula, 10 stool samples and 20 hospital environment swabs), during the period from November 2014 to April 2015 were collected. Growth on Violet red bile agar and MacConkey agar was identified by cultural and biochemical tests and confermated by API 20 E system which revealed that: Only `12/80 (15%) gave positive growth for Enterobacter spp. as following: 3/20 (15%) environmental swabs, 8/50 (16%) infant formula and 1/10 (10%) stool samples. Diagnosis of the isolates appeared that E. cloacae was (6 /12) while E. sakazakii (4 /12) and E. aminogenus (2/12). Four E. sakazakii isolates were screened for their antibiotic resistance against 20 antibiotics of different classes using Kirby-Bauer disk diffusion method. The results showed that all the tested isolates were resistant to at least 6 antibiotics of which they were tested.

Keywords: Enterobacter sakazaki, Infant milk, Stool, Sensitivity, Antibiotics.