Abstract

MOLECULAR IDENTIFICATION OF C. BURNETII BY AMPLIFICATION OF OUTER MEMBRANE PROTEIN (COM1AND COM2) GENES IN ABORTED WOMAN AND SMALL RUMINANTS BY NESTED PCR.

Abdallah K. Hindi*, Mohammed A. Jeber and Abbas D. Mutar

Abstract

Q fever is a worldwide distributed disease caused by Coxiella burnetii bacteria causes several disease main of this disease is abortion, the biological hazard and prevalence of this bacterium on the aborted woman and small ruminants are not known in Iraq. Therefore this study was aimed to detection of Coxiella burnetii as a causative agent of abortion in woman and female of small ruminants. Collected (322) samples includes human samples and animals samples. A total of 120 blood aborted women samples,7 breast milk samples, and 50 blood from normal women as control groups, these samples collected from Bent Al –Huda hospital in Thi – Qar province / Iraq. Animals samples, total of 80 aborted animal blood samples,15 milk samples and 50 blood and 50 serum samples, these samples from normal animals as controls group, these samples collected from veterinary hospital in Thi – Qar province/ Iraq. PCR technique used for DNA identification of C. coxiella in human and animals samples by targeting genes outer membrane protein (com1 and com2), In human blood samples the com1 and com2 genes detected in 23of 120 (19.166%) samples and in breast milk samples 1 of 7 (14.28%). In animals blood samples the com1 and com2 genes detected in 19 of 80 (23.75%) and not detected in milk samples.

Keywords: Coxiella burnetii, C. coxiella, membrane protein.