Abstract

RECENT TRENDS IN HPLC: A REVIEW

Ranjana Suryawanshi*, Avinash Phadtare, Ravi Rajurkar, Manjusha Bhange, Vitthal Lingayat

Abstract

Today, chromatography is the backbone of separation science and is being used in all research laboratories and pharmaceutical industries of the world. In these chromatography techniques, HPLC is one of the chromatographic techniques, which is mostly used analytical technique. The current trend in high performance liquid chromatography (HPLC) tends toward the achievement of higher separation efficiency and shorter analysis time. This article represents a brief review of HPLC along with its principle and instrumentation. It describes about new trends in HPLC such as RRLC, UPLC, UFLC and Nano LC. In this article mainly focus on detailed comparison of new developments of HPLC in terms of instrumental operating conditions such as column temperature, flow rate, injection volume and also cover the applications and advantages over HPLC of each technique.

Keywords: Liquid chromatography, HPLC, RRLC, UPLC, UFLC, Nano LC.