Abstract

EVALUATION OF ANTIUROLITHIATIC ACTIVITY OF RHUS MYSORENSIS AGAINST ETHYLENE GLYCOL INDUCED UROLITHIASIS IN WISTAR RATS

Magulury Mounika*, Raghuveer Rodda, Sushma M & V Uma Maheswara Rao

Abstract

Background and Objectives: Urinary stone disorder has afflicted humankind since antiquity and can persist, with serious medical consequences, throughout a patient’s lifetime. The common component of urinary stone is calcium oxalate (CaOx). In spite of tremendous advances in the field of medicine, there is no truly satisfactory drug for the treatment of renal calculi. In the indigenous system of medicine, the leaves of Rhus Mysorensis are reported to be useful in the treatment of urinary stones. Hence, in the present study, the Rhus Mysorensis have been selected for their antiurolithiatic activity on experimentally induced urolithiatic rats. Materials and Methods: The Rhus Mysorensis were shade dried at room temperature and coarsely powdered in such a way that the material passed through sieve no. 20 and was retained on sieve no. 40 for desired particle size and then extracted with 80 %v/v ethanol, separately. Acute toxicity study was carried out using “Up and Down” method. Male Wistar albino rats were used for assessment of antiurolithiatic activity. Ethylene glycol and ammonium chloride induced hyperoxaluria model was used to induce urolithiasis. Thirty animals were randomly divided into five groups containing six animals in each. Group I served as a vehicle treated control and maintained on regular rat food and drinking water ad libitum. Ethylene glycol (0.75%) in drinking water was fed to groups II-V for induction of renal calculi until the 28th day. As well as ethylene glycol, groups 2-5 also received the following treatments: Groups III received standard antiurolithiatic drug, cystone (750 mg/kg body weight).Group IV received methanolic extract of Rhus Mysorensis (200 mg/kg body weight) Group V received methanolic extract of Rhus Mysorensis (400 mg/kg body weight). Extract was given once daily by oral route. After 28 days, urine 24 h) was analyzed for oxalate, calcium and phosphate excretion.

Keywords: Rhus Mysorensis, antiurolithiatic, ethylene glycol, hyperoxaluria model, urinary stones.