Abstract

COMPARATIVE STUDY OF MAST CELL STABILIZING EFFECT OF EXTRACT OF SELECTED PLANTS WITH PROVEN H1 ANTAGONIST ACTIVITY

Krishna Prasad, Rashmi Rajappa, Jessen George, Shastry C.S, Magesh S. Bojan*

Abstract

Mast cells play a key role in allergy and inflammation which have profound patho-physiological role in asthma. In the present study a comparative effect of mast cell stabilizing activity of different plant extracts Momordica dioica fruit pulp (family - Cucurbitaceae), Cuminum cyminum seeds (family - Apiaceae), Piper nigrum seeds (family - Piperaceae), Boerhaavia diffusa roots (family - Nyctaginaceae.), Withania somnifera roots (family - Solanaceae), Mangifera indica leaves (Anacardiaceae), Plantago ovata (Plantaginaceae) was carried out. For this purpose plant extracts were subjected to the preliminary phytochemical analysis and mast cell stabilizing action by assessing the prevention of degranulation of mast cells by known degranulating compound 48/80. The result showed a higher stabilization potential in ethanolic extract of Piper nigrum seeds which showed 55.17±1.56, 64.00±1.18 and 70.50±0.96 percentage inhibition of mast cell degranulation. Minimal activity was observed in the ethanolic extract of Plantago ovata with percentage inhibition of 21.17±1.54, 22.17±2.21, 24.33±2.20 at three dose levels. Other plant extracts showed intermediate activity. Further investigation of the 5-Lipooxygenase activity, leukotriene antagonist activity and anti-inflammatory activity along with the findings of the present work would give us more clear understanding of the mechanism by which the above extracts exert beneficial effect in the management of asthma.

Keywords: Mast cell, Inflammation, degranulation, compound 48/80.