Abstract

GC-MS ANALYSIS OF ALBIZIA LEBBECK BENTH

Md. Nazneen Bobby, Wesely Edward Gnanaraj, Johnson Marimuthu Alias Antonisamy*, Anto Arockia Raj Adaikalam, Vinnarasi Jamesraj

Abstract

Objective: The present study was aimed to characterize the chemicalprofile present in the petroleum ether, metahnolic, ethylacetate extractof leaves of Albizia lebbeck Benth by using GCMS analysis andpredict their biological activity using PASS. Methods: 10 g powderedsample is soaked with 30 mL methanol, petroleum ether and ethylacetate overnight and filtered through an ash less filter paper withsodium sulphate (2 g). The extract is concentrated to 1 mL by bubblingnitrogen into the solution. 2 μL of the extracts of leaves of A. lebbeckwere employed for GC-MS analysis. The detection employed the NISTVer.2.0-Year 2005 library and Wiley library. The biological activitiesare predicted based on Dr. Duke’s Phytochemical and EthnobotanicalDatabases and Pass prediction. Results: The petroleum ether andmetahnolic, extract of A. lebbeck leaves revealed the presence of 23different compounds whereas ethyl acetate extract of A. lebbeck showed the presence of 25different compounds. PASS analysis predicted various kinds of biological properties for theidentified compounds. Conclusion: It paves the way for the development of several treatmentregimens based on different extracts. Further work is needed to isolate and identify thesebioactive compounds.

Keywords: GC-MS; PASS; Phytochemistry; Bio-efficacy.