Abstract

PREPARATION OF POLYVALENT DIAGNOSTIC ANTISERA FOR SALMONELLA SEROVARES TYPHI AND TYPHIMURIUM

Ameer S. Y. AL-Edhari*, Ala H. Haider, Habeeb S. Naher

Abstract

Polyvalent diagnostic antisera for S.Typhi and S. Typhimurium havebeen prepared during this study according to protocol being suggestedby Florida State University. Salmonella isolates were received fromlaboratory of veterinary College of AL-Qadissya University.Preparation of heat killed s antigen for each isolates were prepared andinjected in three groups of laboratory animals (rabbits). The first andsecond groupwere injected with different antigens in four successivedoses with an interval time of three to four days, while the third groupwas injected with normal saline and used as control. After the lastfourth injection, 10 ml of blood was collected from animals by heartpuncture then serum has been separated by centrifugation. Theseparated antisera was tested with live and killed Salmonella antigens using the agglutinationtest. The results were highly positive and specific. To determine the purity of preparedantisera, it cross reaction with live antigens of some species belong Entrobactrecearepresented by E.coli, Klebsiella Spp. and Protus Spp. The results indicated that there is noconsiderable reactions.

Keywords: S. Typhi, S. Typhimurium, E.coli, Klebsiella Spp. and Protus Spp.