Abstract

‘LYCOPENE LESSENS BLEOMYCIN INDUCED MICRONUCLEI FREQUENCY IN CULTURED HUMAN LYMPHOCYTES IN VITRO’

Shraddha Saha, Sonal Vashi, Dhruti Mistry, Meonis Pithawala* and
Sumitra Chakraborty

Abstract

The cytokinesis-block micronucleus (CBMN) assay is a comprehensive technique for measuring DNA damage, cytostasis, and cytotoxicity in different tissue types, including human lymphocytes. In present study protective effects of lycopene in cultured human lymphocytes against Bleomycin-induced DNA damage in the form of micronuclei (MN) has been reported. Four different cultures were set up. First culture vial was kept untreated, second was treated with lycopene (8μg/ml), third was treated with Bleomycin (15μg/ml) and fourth received combined treatment of lycopene (8μg/ml) and Bleomycin (15μg/ml). Positive implications were recorded about protective effects by lycopene on Bleomycin induced DNA damage in the form of micronuclei (MNi), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs).

Keywords: Lycopene, Bleomycin, Micronuclei Assay.