Abstract

CHEMICAL INVESTIGATION OF FATTY ACID, PHENOLIC CONTENTIN ARACHIS HYPOGAEA, ANACARDIUM OCCIDENTALE, PRUNUS DULCIS, PRUNUS ARMENIACA AND COMPARISON OF THEIR ANTIBACTERIAL ACTIVITY WITH AMOXICILLIN

*Agrawal Shelly, Malviya Shikha and Shukla Rudra Narayan

Abstract

Polyphenols are the widely distributed secondary metabolites in plants showing potent antioxidant properties against pathogenic bacteria, thus their identification and analysis has become a major area of research.The present study reveals the polyphenolic composition and antibacterial activity found in Arachis hypogaea, Anacardium occidentale, Prunus dulcis, Prunus armeniaca seeds. The phytochemical analysis of methanolic extract shows the presence of alkaloids, glycosides, tannins, phenolic group, flavonoids sterols. Quantitative analysis of vitamins shows that being rich in vitamin B complex, have high nutritional value. The total phenolic content determined by colorimeter reveals that P.K, C.K, A.K., Apr .S. Contain 59 mg/gm, 24 mg/gm, 22 mg/gm, 82 mg/gm GAE respectively whereas individual compounds detected by GC-MS technique shows the presence of d-Glycero-d-galacto-heptose, α-D-Galactopyranose, 6-O-(trimethylsilyl)-, cyclic 1,2:3,4-bis (methylboronate), Hexadecanoic acid, methyl ester, n-Hexadecanoic acid, 6- Octadecenoic acid, methyl ester, Oleic Acid ,13-Docosenamide, (Z)- in Arachis hypogeae, Cyclodecasiloxane, eicosamethyl-, Hexadecanoic acid, methyl ester, Estra-1,3,5(10)-trien- 17β-ol, Cyclodecasiloxane, eicosamethyl-, 9-Octadecenamide, (Z)-,2,3-Dimethoxy-5-methyl- 6-dekaisoprenyl-chinon, Cyclodecasiloxane, eicosamethyl inAnacardium occidentale, 4H- 1,2,4-Triazole-3-thiol, 4-methyl-5-[5-(1-pentynyl)-3-pyridyl]-, Pentadecanoic acid, 14- methyl-, methyl ester, 10-Octadecenoic acid, methyl ester, 9-Octadecenamide, (Z)- in Prunus dulcis, Cyclodecasiloxane, eicosamethyl-, Pentadecanoic acid, 14-methyl-, methyl ester, Dibutyl phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, 9-Octadecenamide, (Z)-,7-(4-Methoxy-phenyl)-cyclohepta-1,3,5-triene, Octasiloxane, 1,1,3,3,5, 5,7,7,9,9, 11,11, 13,13,15,15-, hexadecamethyl- in Prunus armeniaca respectively. The antimicrobial activity of methanolic extract against gram positive bacteria when compared with amoxicillin reveals that they exhibit good antibacterial property and thus can be used for therapeutic purpose in future.

Keywords: Arachis hypogaea, Anacardium occidentale, Prunus dulcis, Prunus armeniaca, methanolic extract, vitamin analysis, total polyphenolic content, antibacterial property, amoxicillin GC-MS, UV Spectrophotometer.