Abstract

ANALYSIS OF ANTIBIOGRAM OF PSEUDOMONAS AERUGINOSA FROM VARIOUS CLINICAL SPECIMENS WITH SPECIAL REFERENCE TO MDR – CAUSE FOR CONCERN

Nithyalakshmi J.*, Mohanakrishnan and Sumathi G.

Abstract

Development of antimicrobial resistance by Pseudomonas has been progressive and relentless that renders existing antibiotics obsolete. A great challenge exists if there is an emergence of MDR pseudomonas as clinicians are left with limited therapeutic options; we aimed to investigate the susceptibility pattern of P. aeruginosa isolates in our settings and to do analysis of antibiogram from various clinical samples with special reference to MDR P. areuginosa isolates. This study was designed to conduct in a tertiary care teaching hospital in Chennai over a period of one year. Different types of clinical specimens such as Sputum, Urine, Pus, Throat swab, Eye swab, Ear swab, High vaginal swab (HVS). etc received were analyzed. All isolates of Pseudomaons aeruginosa were identified by standard microbiological procedure and subjected for antipseudomonal antibiotic susceptibility by Kirby bauer disc diffusion method as recommended by CLSI guidelines. Results obtained showed the isolation rate of Pseudomaons aeruginosa was 6.7% (142/2119). The maximum isolates (63/142) were obtained from pus/wound swab which accounts for 44.36% of total. We observed 99.29% of isolates were sensitive to Imipenam followed by combination drug, Piperacillin and Tazobactam (90.84%). And Amikacin (72.53%).A total of 21 isolates were resistant to three or more of the antibiotics, thus MDR (Multi Drug Resistamt) rate was found to be 14.78%. All MDR isolates were sensitive to Imipenam except one obtained from pus. This study emphasizes the need for continuous monitoring of Pseudomonas antibiogram to provide the clinicians the up to date knowledge about the emergence of resistance, if any.

Keywords: Pseudomaons aeruginosa, Tazobactam and Amikacin.