Abstract

ANTIMICROBIAL ACTIVITY AND IN VITRO ANTICARCINOGENIC PROPERTIES OF ‘PORTULACA QUADRIFIDA LINN’ON COLON CANCER USING DIFFERENT EXTRACTS

*Shaik Mohd Khasim and Dr. E. P. Kumar

Abstract

We are living in the country where we finds a tremendous plant sources used for various treatment of diseases. Portulaca quadrifida is one among the Indian plant found in tropical parts. Portulaca quardifida Linn (portulacaceae) is traditionally used for the treatment against various ailments which is scientifically not revealed. In the present study chloroform extract (CHCL3), methanol extract (CH3OH) and aqueous extract of Portulaca quadrifida on human colon cancer cell lines HT-29 is investigated by MTT assay and also plant was screened for its antimicrobial potential by agar well diffusion method. By comparing all the three extracts, aqueous extract showed the highest inhibition with the lowest concentration of 15.87 μg/ml. and it is comparatively safer when compare to other extracts as it is easily eliminated from the body. The antibacterial activity was measured by nutrient agar method and antifungal activity by potato dextrose agar method was noticed with all the three extracts. Among all the three extracts aqueous extract showed significant activity by inhibiting Gram negative bacteria Escherichia coli, Gram positive bacteria Staphylococcus aureus, and fungal organism like Aspergillus flavus and Aspergillus niger at MIC of 4.3, 4.2, 4.4 and 3.7 at 25mcg/ml, respectively. The anti cancer and antimicrobial effects of Portulaca quadrifida may be related to their content like flavonoids, saponins and tannins.

Keywords: Portulaca quadrifida, Colon cancer, Antimicrobial activity, In vitro.