Abstract

ISOLATION OF PSEUDOMONAS AERUGINOSA FROM INDUSTRIAL WASTE WATER USING LIPA GENE

Wathiq A. Al-Daraghi, Intissar Taha and Essam F. A. Al- Jumaily*

Abstract

Extracellular lipase producing by Pseudomonas aeruginosa have special properties not common among the lipases produce by other organisms, e.g., their thermorestance and activity at alkaline pH. Which make this enzyme have considerable value for use in numerous applications in many industries and in degrade oils in industrial wastewater. This study aimed to use PCR technique for isolation P. aeruginosa from industerial wastewater by lipA gene and to find the suitable strain which have the highest lipolytic activity by subjected the PCR product to BLAST (Basic Local Alignment Research tool) analysis, to use this suitable strain in oil rich industrial wastewater treatment and in many industrial applications by using lipase that produced from it.

Keywords: Pseudomonas aeruginosa; industrial waste water; PCR.