Abstract

INCREASING (PHENOLYIC AND FLAVONIODS COMPOUNDES OF CICER ARIETINUM L. FROM EMBRYO EXPLANT USING TITANUM DIOXIDE NANOPARTICLE IN VITRO

*Hashim K. Mohammed AL-oubaidi and Nada M. Kasid*

Abstract

The present study is aimed to increase the secondary metabolite production (phenolic and flavonoids compounds) in Cicer arietinum L.invitro. Secondary metaboliteswas estimated qualitatively and quantitatively using high performance liquid chromatography HPLC compared with the mother plant. In order to increase the production of secondary metabolites, titanium dioxide nanoparticle used at concentrations (con, 0.5,1.5,3,4.5,6) mg/l .The results revealed that the concentrations (4.5,6) mg/l TiO2 nanoparticle caused highly significant production in most of the secondary metabolites from callus embryo of Cicer arietinum L.

Keywords: callus induction, plant tissue cultures, Cicer arietinum, secondary metabolites, titanium dioxide nanoparticle.