Abstract

ANTI-CANCER ACTIVITY OF GREEN SPORAL POLYKETIDE FROM STREPTOMYCES SP-JB87 ISOLATED FROM JASMONIUM BREVILOBUM

Subhash Chandra Bose Veluswamy, Parimala Gnana Soundari Arockiam, Jabastin Jeyamanohar, Suresh Rajendran and Dr. Brindha Priyadarisini Venketasen*

Abstract

Actinomycetes were isolated from rhizosphere soil of medicinal plants from Dodabetta forest and 8 strains were selected based on unique aerial spore pigment and morphology. Aerial spores were harvested from actinomycetes grown on solid state media. DMSO-Ethylacetate extraction and precipitation of Sporal polyketide pigments were made and screened for its in vitro anti-cancer activity against A549 cell lines. The selected green sporal polyketide (GSP) pigment was purified by Fractional precipitation and two distinct fractions were observed in HPLC-PDA detector system. FTIR and UV-Visible spectral analysis revealed the presence of protomelanin like polyketide precursors responsible for melanin formation in aerial spore walls. The GSP extracted from Streptomyces sp. JB87 isolated from Jasmonium brevilobum showed EC50 value of 70 μg/ml against A549 lung cancer cell lines.

Keywords: A549 cell lines; Green Sporal Polyketide (GSP); FTIR; HPLC; Protomelanin; Streptomyces sp-JB87.