Abstract

OPTIMIZATION OF EXTRACTION CONDITIONS AND FINGERPRINT DEVELOPMENT OF LEUCAS ASPERA LINN. BY HPTLC AND HPLC

Ajitkumar BS*, Prashant Hande, Shailendra Rane and Manish Hate

Abstract

Leucas aspera Linn. has been used in ayurvedic medicine for treatment of various ailments. An attempt has been made to optimize different extraction conditions for leucas aspera linn. Hence, it helps for the extraction of the phytochemical constituents and which is used for standardization of chromatographic techniques. Chromatographic fingerprint analysis of herbs and herbal drugs represents a comprehensive qualitative approach for the purpose of species authentication, evaluation of quality and ensuring the consistency and stability of herbs, herbal drugs and their related products. Therefore, aim of this study was to develop the high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) fingerprint profile of dried whole plant extract of leucas aspera linn. In the present research article HPTLC and HPLC fingerprint profile of raw material from whole plant of leucas aspera linn. in methanolic extract. HPTLC and HPLC method for the separation of active phytochemical constituents in leucas aspera linn. extracts has been developed. For HPTLC of these extracts was performed on silica gel 60F254 by semiautomatic applicator and using solvent system toluene: ethyl acetate: methanol (7:2:1, v/v/v) and for HPLC mobile phase A: 0.1% formic acid in water and B:acetonitrile were used. Hence, it was concluded that the established fingerprint provide theoretical and technical support for routine quality control, species identification, authentification and was appropriate for standardization of drug.

Keywords: leucas aspera linn, Extraction, CAMAG HPTLC, Shimadzu HPLC, Fingerprint, authentification, standardization.