Abstract

ANTIBACTERIAL ACTIVITY OF ARECA CATECHU L. FRUIT EXTRACT LOADED SILVER NANOPARTICLES

Foysal Hossen, Md. Mamun Or Rashid*, Mohammad Morshad Alam, Mir Salma Akter, Md. Tanvir Hossain, Kazi Nahid Akhter

Abstract

Silver nanoparticles are synthesized by AgNO3 as a precursor and NaBH4 was used as reducing agent. Here we tried to investigate the antibacterial activity of polyaniline coated silver-extract nanoparticles using Areca catechu L. fruit extract. Prepared nanoparticles were characterized by Visual inspection, Ultraviolet-visible spectroscopy, Scanning Electron Microscopy (SEM) techniques. Antibacterial activities of the synthesized Ag-Extract nanoparticles were tested against Staphylococcus aureus ATCC 25923, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. UV–Vis spectrum of reaction mixture showed strong absorption peak with centering at 400 nm. SEM results revealed that the particle size of these combined nanoparticles were 78.5 to 100 nm in most cases. The result revealed that Areca catechu L. coated Ag-Extract NPs showed strong antibacterial activities against most of the tested species. 34±0.68 mm zone of inhibition was found against S. aureus, whereas it was 24±0.40 mm for the standard ciprofloxacin (positive control). Again, maximum zone of inhibition 19±0.30 mm was found for S. typhimurium, 35±0.48 mm for E. coli and 29±0.35 mm for P. aeruginosa. This result suggests that S. typhimurium is more resistant than E. coli and P. aeruginosa. In-vivo investigation is needed on human model before establishment of antibacterial efficacy of AgNPs-extracts (A. catechu) in drug development program for safe health care services.

Keywords: Silver nanoparticles, Areca catechu L., Antibacterial activity, Scan Electronic Microscopy (SEM).