Abstract

FORMULATION AND IN VITRO EVALUATION OF SOLID DISPERSIONS OF METHANOLIC EXTRACT OF VERNONIA AMYGDALINA- PEG-4000: A WOUND HEALING STUDY

*Momoh, M. A. Kenechukwu, F.C Agboke, A. A, Akpabio, E. I and Adedokun, M. O

Abstract

Objective: Preparation containing V. amygdalina-PEG solid dispersion SDs was evaluated for wound-healing potential on different experimental models of wounds in rats. Methods: Vernonia amygdalina solid dispersions SDs containing varying concentrations 0:1, 1:0 1:1, 1:3 and 3:1, of Vernonia amygdalina: PEG 4000 were prepared using the fusion -solvent evaporation method. Wound contraction ability in excision wound model was measured at different time intervals and study was continued until wound is completely healed. The effects of the preparation on the activities of liver were similarly assessed. Results: Tensile strength was measured in 9th -dayold incision wound. Preparation (d) containing 1:3 of PEG : V. amygdalina showed statistically significant response, in terms of wound contracting ability, wound closure time, period of epithelization, tensile strength of the wound, when compared with the individual components and the control group (negative control), the results were comparable to those of a commercial neomycin formulation (positive control). The liver parameters and hematological studies did not show much variation to that of the control. Conclusion: It is believe that SDs of this extracted could be formulate into pharmaceutical dosage form and used as an alternative in wound healing.

Keywords: Soilid dispersion, PEG-4000, wound, V. amygdalina.