Abstract

VANCOMYCIN RESISTANCE IN STAPHYLOCOCCUS EPIDERMIDIS CLINICAL ISOLATES

May T. Flayyih* and Mariam K. Abdrabaa

Abstract

Minimum inhibitory concentrations of vancomycin to thirty Staphylococcus epidermidis isolates were determined . The results revealed that (12) S.epidermidis isolates ( 40 %) were vancomycin resistant, the MIC of them were between 256 μg\ml and 32 μg\ml , (4) S.epidermidis isolates (13.3%) were intermediate resistance , the MIC to 3 of them were 16 μg\ml and the last was 8 μg\ml. The isolates were subjected to polymerase chain reaction (PCR) technique in monoplex pattern to amplify resistant incoding the vanA and vanB genes. The results by this study showed that 12 (40%) S. epidermidis isolates gave the implicone size (1030 base pair) of the vanA gene. However the results of MIC and PCR were similar but no any isolates gave product for presence of vanB gene.

Keywords: Staphylococcus epidermidis, vancomycin resistant, vanA gene, vanB gene.