Abstract

GROWTH HORMONE GENE IN IRAQI AND TURKISH AWASSI SHEEP USING PCR-RFLP

Luma A. Othman*, Amina N. Althwani Abdul Jabbar A.H. Alkhazraji

Abstract

The objective of the study was to examine the polymorphic GH/HaeIII gene in Awassi sheep breed (Iraqi and Turkish) Eighty genomic DNAs that consisted from 40 Iraqi and 40 Turkish Awassi sheep were isolated from blood samples of those animals. A fragment of GH/HaeIII gene, comprising of a part of intron 2, complete exon 3, complete intron 3, complete exon 4 and a part of intron 4, was amplified. The amplified product with the length of 934 bp was digested with HaeIII restriction enzymes and in Turkish Awassi showed the presence of GH HaeIII homozygotes AA genotype, with fragments 277, 202, 110, 100, 94, 68, 49 and 22,8,4 did not appeare on agarose gel under Uv light, Homo And heterozygous genotype with fragments 277,256,202,110,100,94,68,49bp showed in Iraqi Awassi sheep. The present study concluded that GH/HaeIII could be a genetic marker in sheep for improvement the purity of the Turkish breed. And diversity in the Iraqi Awassi sheep.

Keywords: Awassi sheep, HaeIII enzyme, homozygosity, hetrozygosity, growth hormone.