Abstract

POSSIBLE DETECTION OF CAG GENE IN ESCHERICHIA COLI STRAINS ISOLATED FROM PATIENTS WITH UTI

Yasmeen Reyadh Saeed*, Mohammad Sabri Abd-ALRazzaq and Mohammad Redha Joudy.

Abstract

In this study, (100) urine specimens obtained from patients suffering from Urinary Tract Infection and also, 25 stool samples were taken from healthy individual as control group were included, from both sexes who admitted to Al- Hilla Teaching Hospita during a period from February (2015) to May (2015). The samples were then cultured on selective media to investigate on Escherichia coli. Out of the 100 urine samples, only 80 samples showed positive growth and for about 40 isolates of E.coli were isolated where 10 (25%) of the isolates from male patient and 30 (75%) from female patients. Moroever, 7 isolates E.coli were isolated from stool samples of human individual. Molecular detection of cytotoxic associated gene A ( cagA ) was done to show the sequence homology of this gene in H.pylori to that in E.coli by using PCR technique. The results showed that all isolates of E.coli from urine and stool gave negative result for this marker in E.coli which confirm that there is no sequence homology of this gene with that present in H. pylori. The same results were seen with cytotoxic associated gene E ( cagE )when specific marker from H. pylori is used. Besides, the isolates of E. coli were subjected to show their possession of virBII gene, by using specific marker. It was observed that 25 (62.5%) of bacterial isolates from urine and 5 (71.4%) of bacterial isolates from stool gave positive result for this gene, which is not previously detected in E. coli. This result indicate the presence of sequence homology of this gene with that present in H. pylori.

Keywords: Al- Hilla, H.pylori, E.coli,