Abstract

EVALUATION OF CYTOTOXIC POTENTIALITY OF DIFFERENT EXTRACTS OF GLOCHIDION VELUTINUM WIGHT’S LEAVES THROUGH BRINE SHRIMP LETHALITY BIOASSAY

Mohammad Raquibul Hasan, Tufikul Islam*, Md. Iqbal Hussain Shameem, Md. Abdul Awoal, Md. Shafiqul Islam, Md. Sohel Rana

Abstract

The aim of the work was to investigate the cytotoxic potentiality of leaf of G. velutinum through Brine shrimp lethality bioassay (BSLA). Methanol, Ethyl Acetate and n – hexane extracts of G. velutinum leaf was investigated on eggs of Artemia salina. Various phytochemical constituents like carbohydrates, glycosides, glucosides, alkaloids, glycosides, steroids, tannins, flavonoids and saponin were found in preliminary phytochemical screening. In Brine shrimp lethality bioassay (BSLA), methanol, ethyl acetate and n-hexane extract has cytotoxic activity with LC50 of 428.47μg/ml, 651.92 μg/ml and 598.54μg/ml respectively. So extensive research work is necessary to find out the active constituent responsible for this activity.

Keywords: Brine shrimp lethality bioassay (BSLA), Dimethylsulfoxide (DMSO), cytotoxicity.