Abstract

FAST AND SENSITIVE VALIDATED METHOD FOR QUANTITATION OF HALOPERIDOL IN HUMAN PLASMA USING ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) WITH DIODE ARRAY DETECTION

M. Siva Selva Kumar and M. Ramanathan*

Abstract

A simple, sensitive and rapid ultra-performance liquid chromatography (UPLC) method has been developed and validated for estimation of haloperidol (HAL) in human plasma in vitro. Sample was prepared by simple liquid-liquid extraction technique. Chromatographic separation of HAL and IS was achieved by using Acquity UPLC BEH shield RP18 column and maintained at 35ºC temperature. The mobile phase consist of a mixture of 12 mM ammonium acetate buffer pH 3.5 adjusted with acetic acid (80%, v/v) and acetonitrile (20%, v/v) at a flow rate of 0.4 mL/min. Quantification was carried out on a photodiode array detector set at 240 nm. HAL and IS retention time were found to be 1.8 and 1.4 respectively. The total run time was 2.5 min. The method was validated for specificity, selectivity, recovery, accuracy, precision, recovery and stability. The calibration curve was linear the concentration range of 1 to 100 ng/mL. The method can be employed in therapeutic drug monitoring practices and clinical toxicological assays.

Keywords: UPLC; Haloperidol; Therapeutic drug monitoring; human plasma.