Abstract

PHARMACOLOGICAL EVALUATION OF TUMOUR INCIDENCE, APOPTOSIS AND HISTOPATHOLOGICAL INTERPRETATION OF NEOPLASTIC LESIONS WITH DAIDZEIN A PHYTOESTROGEN IN DMBA INDUCED MAMMARY TUMOURS

*Dr. Vanitha Samuel, Dr. Parthasarathy Nirmala and Dr. N. Chidambaram

Abstract

Phytoestrogens as potential sources of chemo prevention in mammary carcinogenesis were proved in our earlier studies. Daidzein, a phytoestrogen was used to explore its anti - cancer potential using Wistar rats models and to evaluate its apoptotic activity in mammary carcinogenesis induced by 7, 12-dimethylbenz (a) anthracene (DMBA). This chemical carcinogen was administered (25mg/rat/s.c) in divided doses to induce mammary cancers in female wistar rats. Daidzein 50mg/kg; 100mg/kg; Tamoxifen as reference standard (40mg/kg) was administered, from the first day of the study. The growth of tumors was monitored. The tumour incidence, tumour volume, histopathological analysis and macroscopic changes showed that neoplastic lesions in the drug treated groups was comparatively reduced and their mortality lesser than the DMBA treated groups, at the end of the study.

Keywords: Chemoprevention, Daidzein, DMBA, histopathological analysis, macroscopic changes, mammary carcinogenesis, phytoestrogens, Tamoxifen, tumour incidence and tumour volume.