Abstract

EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT OF ETHANOL AND AQUEOUS EXTRACTS OF POLYHERBAL FORMULATION

Ajay Mandal* and P. Jagan Mohan Reddy

Abstract

To evaluate the in-vitro antioxidant potential of polyherbal preparation, a combination of six selected medicinal plants. The antioxidant potential of the aqueous extract and ethanolic extract of Ocimum sanctum, Withania somnifera, Gymnema sylvestre, Tinospora cordifolia, Phyllanthus emblica and Coleus forskohlii. Antioxidant potential was investigated by using in vitro assay like ABTS radical scavenging assay, DPPH radical scavenging activity and Total phenolic content. The effects were related with standard (Gallic acid), a known antioxidant. Various Phytoconstituents identified in the above selected plants extracts were poly phenols, flavonoids, terpenoids, tannins, alkaloids. The terpenoids were stated to possess the scavenging properties against the free radicals. The presence of these Phytoconstituents in selected plants might be responsible for antioxidant activity; gallic acid is used as a standard. Conclusion: Polyherbal formulation is better than individual plant extracts.

Keywords: Polyherbal formulation (PHF), phytochemicals, Anti-oxidant activity and Gallic acid.