Abstract

SCREENING OF ANTIMICROBIAL ACTIVITY OF CRUDE EXTRACTS OF CARICA PAPAYA LEAF AGAINST PATHOGENIC BACTERIA

Pragya Pandey*, F. C. Garg and Lokesh K. Tripathi

Abstract

Nowadays, production of plant-based-drugs increases for the treatment of many diseases. The present study investigates the presences of antimicrobial activity and phytochemical compounds of Carica papaya leaf extracts. The leaf extract was prepared using five organic solvent (petroleum ether, chloroform, ethyl acetate, ethanol, and methanol) and water by maceration method. The phytochemical analysis of resulted extracts confirm the presences of reducing sugar, alkaloids, flavanoids, tannin, steroids and terpenoids. The antimicrobial nature of the resulted extracts were studied by agar well diffusion method against infectioncausing pathogens viz., Escherichia coli, Staphylococcus aureus, Micrococcus luteus, Salmonella enterica, Acenetobacter and Pseudomonas aeruginosa. Methanol leaf extract revealed the pronounced antibacterial effect with 19mm inhibition zone against on Salmonella enterica. Ethyl acetate extract showed their activity with inhibition zone of 17mm against P.aeruginosa. Maximum MIC value was found between 5- 25μg/ml. The results obtained were justified that papaya leaves contain some active antimicrobial compounds which may be used as an antimicrobial agents to treat the infection as a traditional practice.

Keywords: Carica papaya, antimicrobial activity, phytochemical constituent, MIC.