Abstract

ANTI-INFLAMMATORY ACTIVITY OF LECTIN PURIFIED FROM MORUS NIGRA AGAINST LIPOPOLYSACCHARIDE (LPS) INDUCED RENAL STRESS IN RATS

*Youcef Necib and Ahlem Bahi

Abstract

The study was designed to investigate the possible protective role of lectin of Morus nigra in lipopolysaccharide renal inflammatory, by using biochemical approaches. The effects of lectin of Morus nigra on LPS induced oxidative and renal stress were evaluated by serum creatinine, urea and uric acid levels, kidney tissue lipid peroxidation, GSH levels, SOD, GSH-Px , GST and catalase activities. Administration of LPS induced significant increase in serum: creatinine, urea and uric acid concentration showing renal inflammatory. LPS also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, SOD, GSH-Px, GST and catalase activities along with increase the level of lipid peroxidation. Furthermore, treatment with LPS caused a marked elevation of kidney weight and decreased body weight. lectin of Morus nigra treatment markedly reduced elevated serum: creatinine, urea and uric acid levels and conteracted the deterious effects of LPS on oxidative stress markers and attenuated histological changes caused by LPS in kidney. Our results indicate that lectin of Morus nigra could have a beneficial role against LPS induced nephrotoxicity and oxidative stress in rat.

Keywords: Antioxidant enzymes, LPS, lectin, Morus nigra, renal inflammatory.