Abstract

ADVANCES ON ACUTE CARBAMAZEPINE EXPOSURE IN ADULT ZEBRAFISH: PROOF-OF-CONCEPT IN EVALUATION OF ANTIOXIDANT AND ACETYLCHOLINE ESTERASE ENZYMES ACTIVITY

Ashok D*, Renuka Jyothi S, Dr. B.P. Harini, Manasvi Vukku, Sneha Venkatachalapthy and Prarthana V Kowsik

Abstract

Carbamazepine is a drug for the treatment of seizure disorders and neuropathic pain. It may be used off-label as a second line treatment for bipolar disorder. This study aims at use of carbamazepine and its effects on various organs and tissues of adult zebrafish and its related antioxidant enzymes like Super Oxide Dismutase and Catalase and Acetylcholine esterase enzyme. The LC 50 for carbamazepine was determined as 50ppm. As the concentration of drug dose was increased the activity of various enzymes was seen to increase exponentially. The activity of SOD was highest in brain at both 10ppm and 5ppm followed by muscle and gill and had significant increase against control fishes. Similarly, the activity of catalase and acetylcholine esterase was highest in brain at both 10ppm and 5ppm followed by gill and muscle which had a significant increase against untreated zebrafish. The observation in this research reflects that all the enzymes activities were significantly enhanced in all the tissues (Gill, Brain, Muscle) when compared to CBZ unexposed ones. This obtained result could be due detoxification mechanism exhibited by the Danio rerio on exposure to CBZ.

Keywords: Carbamazepine, Danio rerio, Super Oxide Dismutase, Catalase, Acetlycholine esterase.